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Research Associate, DNA & Strain Construction (Contractor)

Asimov

Asimov

Boston, MA, USA
Posted on Apr 25, 2025
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Our mission at Asimov is to advance humanity's ability to design living systems, enabling biotechnologies with outsized benefit to society. We're developing a mammalian synthetic biology platform––from cells to software––to enable the design and manufacture of next-generation therapeutics.
We are looking for a highly motivated Research Associate I to join our DNA & Strain Construction (DNASC) team on a contract basis, with the potential for full-time hire after six months. In this role, you will support our high-throughput molecular biology pipeline, contributing to the efficient cloning, assembly, and sequencing of DNA constructs. You will gain hands-on experience with cutting-edge molecular biology techniques, automation, and process optimization while working in a dynamic synthetic biology environment.

About the Role:

  • Perform high-throughput cloning workflows, including PCR, Gibson Assembly, and Golden Gate Assembly to construct plasmids.
  • Operate liquid handlers (e.g., Echo, Bravo, Hamilton STAR), run the MiSeq for NGS, and utilize other automation tools for NGS library preparation and molecular cloning.
  • Conduct PCR, Golden Gate or Gibson Assembly, plasmid purification, and sequencing prep to validate DNA constructs.
  • Maintain accurate sample tracking and documentation in LIMS to ensure data integrity and reproducibility.
  • Assist with large-scale plasmid preparations (LSPs) to support internal and external projects.
  • Collaborate with scientists and other RAs to troubleshoot workflows and improve efficiency.
  • Follow standard operating procedures (SOPs) and contribute to continuous process improvements.
  • This role will begin as a full-time, 6 month contract. Dependent on business needs, there is potential for the contract to be extended or converted into a permanent position.

About You:

  • B.S. in Biology, Biochemistry, Bioengineering, or a related field (recent graduates encouraged to apply).
  • Hands-on experience with molecular cloning techniques (PCR, restriction digestion, ligation, transformation, and plasmid purification).
  • Familiarity with DNA assembly methods (Golden Gate, Gibson) and bacterial culturing techniques.
  • Comfortable working in a fast-paced lab environment, following protocols, and maintaining detailed records.
  • Experience with automated liquid handlers (Echo, Bravo, Hamilton STAR) or NGS workflows is a plus.
  • Highly organized, detail-oriented, and able to work independently or as part of a team.
We're fueled by a vision to transform biological engineering into a fully-fledged engineering discipline. Should you join our team, you will grow with a constantly evolving organization and push the frontiers of synthetic biology. Company culture is key to Asimov, and ours is a culture of recombination; we believe that our mission can only be achieved by bringing together a diverse team with a mixture of backgrounds and perspectives.
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